Table 2.

Min protein interactions observed by the yeast two-hybrid system

DNA binding domain	Activaiton domain	Interaction*
MinC	−	−
MinD	−	−
MinC	MinD	++++
MinD	MinC	++
MinC19	−	−
MinC19	MinD	++++
MinD	MinC19	++

^*Represents the strength of interaction based on the color development by using X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactoside). ++++, strong interaction and was previously measured at 160 units of β-galactosidase activity (23); +++, intermediate strength; ++, moderate strength and was previously measured at 48 units of β-galactosidase activity (23); + weak activity; −, no interaction because no color development was observed after 24 hr.