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. 1999 Dec 21;96(26):14831–14836. doi: 10.1073/pnas.96.26.14831

Figure 1.

Figure 1

The HIC-1 and γFBP-B BTB/POZ domains are able to homo- and heterodimerize in the mammalian two-hybrid assay. The HIC-1 and γFBP-B BTB/POZ domains were fused either to the GAL4 DNA-binding domain or to the VP16 activator domain. Luciferase and β-gal assays were performed on total extracts from HeLa cells that have been transfected with 750 ng of the GAL4 reporter gene, pG5LUC (CheckMate mammalian two-hybrid kit, Promega), 100 ng of the indicated bait- and activator-tagged expression constructs, and 50 ng of the pSG5 β-gal construct as a control for transfection efficiency. Results represent the average of a triplicate experiment in which the luciferase activity was normalized to β-gal activities.