Abstract
When glycerol-requiring auxotrophs of Bacillus subtilis are deprived of glycerol, the synthesis of fatty acids continues at an apparent rate of 20 to 50% that of supplemented cultures. The newly synthesized fatty acids are not incorporated into phospholipid and accumulate as free fatty acids. These molecules undergo a much more rapid turnover than phospholipid fatty acids, and the rate of turnover is sufficient to indicate that the rate of fatty acid synthesis in glycerol-deprived cultures is similar to that in supplemented ones. The average chain length of the free fatty acids is greater than that of the phospholipid fatty acids. Cells deprived of required amino acids also show a diminution in the apparent rate of fatty acid synthesis; however, in this case, the fatty acids accumulate in phospholipid, and no increase of the free fatty acid fraction is observed. It is argued on the basis of these findings that the control of lipid synthesis does not operate at the level of transacylation but must act on one or more of the reactions of the fatty acid synthetase.
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- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Esfahani M., Ioneda T., Wakil S. J. Studies on the control of fatty acid metabolism. 3. Incorporation of fatty acids into phospholipids and regulation of fatty acid synthetase of Escherichia coli. J Biol Chem. 1971 Jan 10;246(1):50–56. [PubMed] [Google Scholar]
- GOLDFINE H., BLOCH K. On the origin of unsaturated fatty acids in clostridia. J Biol Chem. 1961 Oct;236:2596–2601. [PubMed] [Google Scholar]
- Hsu C. C., Fox C. F. Induction of the lactose transport system in a lipid-synthesis-defective mutant of Escherichia coli. J Bacteriol. 1970 Aug;103(2):410–416. doi: 10.1128/jb.103.2.410-416.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KANEDA T. Biosynthesis of branched chain fatty acids. II. Microbial synthesis of branched long chain fatty acids from certain short chain fatty acid substrates. J Biol Chem. 1963 Apr;238:1229–1235. [PubMed] [Google Scholar]
- KANEDA T. Biosythesis of branched chain fatty acids. I. Isolation and identification of fatty acids from Bacillus subtilis (ATCC 7059). J Biol Chem. 1963 Apr;238:1222–1228. [PubMed] [Google Scholar]
- Majerus P. W., Vagelos P. R. Fatty acid biosynthesis and the role of the acyl carrier protein. Adv Lipid Res. 1967;5:1–33. [PubMed] [Google Scholar]
- Mindich L. Induction of Staphylococcus aureus Lactose Permease in the Absence of Glycerolipid Synthesis. Proc Natl Acad Sci U S A. 1971 Feb;68(2):420–424. doi: 10.1073/pnas.68.2.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mindich L. Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. J Mol Biol. 1970 Apr 28;49(2):415–432. doi: 10.1016/0022-2836(70)90254-8. [DOI] [PubMed] [Google Scholar]
- Osborn M. J. Structure and biosynthesis of the bacterial cell wall. Annu Rev Biochem. 1969;38:501–538. doi: 10.1146/annurev.bi.38.070169.002441. [DOI] [PubMed] [Google Scholar]
- Overath P., Pauli G., Schairer H. U. Fatty acid degradation in Escherichia coli. An inducible acyl-CoA synthetase, the mapping of old-mutations, and the isolation of regulatory mutants. Eur J Biochem. 1969 Feb;7(4):559–574. [PubMed] [Google Scholar]
- Sokawa Y., Nakao E., Kaziro Y. On the nature of the control by RC gene in e. coli: amino acid-dependent control of lipid synthesis. Biochem Biophys Res Commun. 1968 Oct 10;33(1):108–112. doi: 10.1016/0006-291x(68)90263-5. [DOI] [PubMed] [Google Scholar]
- Willecke K., Mindich L. Induction of citrate transport in Bacillus subtilis during the absence of phospholipid synthesis. J Bacteriol. 1971 May;106(2):514–518. doi: 10.1128/jb.106.2.514-518.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wuthier R. E. Two-dimensional chromatography on silica gel-loaded paper for the microanalysis of polar lipids. J Lipid Res. 1966 Jul;7(4):544–550. [PubMed] [Google Scholar]