Figure 3.

FADD and caspase-8 are essential for FasL-induced apoptosis. SKW6, CH1, Jurkat, and CEM cells were stably transfected with expression constructs encoding a FLAG-tagged dominant-interfering mutant of FADD (FADD-DN), a vector encoding FLAG-tagged CrmA, or with a control vector. Expression of the proteins was determined by anti-FLAG staining. Staining of parental cells is shown by the filled histograms (A). Cell lines, thymocytes and purified resting or activated T cells from control, lpr or FADD-DN transgenic mice were cocultured with Neuro2A-FasL or control cells (B) or with 100 ng/ml recombinant FasL + 1 μg/ml anti-FLAG (C). Cell viability was determined after 1–4 days. Data shown represent arithmetic means ± SD of more than or equal to three independent experiments.