Figure 5.

Bcl-2 does not inhibit FasL-induced apoptosis within the whole animal. (A) Thymi from bcl-2 or FADD-DN transgenic or control mice were injected with 10 μg of recombinant FasL + 20 μg of anti-FLAG (filled bars) in one lobe or with anti-FLAG alone in the other lobe (open bars). Mice were killed after 20 hr, and the cellularity of each lobe was determined. Data shown represent arithmetic means ± SD from three mice of each genotype. Mice expressing a bcl-2 transgene in hepatocytes were injected iv with 12 μg of recombinant FasL + 25 μg of anti-FLAG and killed when moribund (B). Serum levels of the liver enzymes alanine aminotransferase or aspartate aminotransferase (C) were measured 45–100 min after the injections. (D) Representative hematoxylin/eosin-stained liver sections (magnification, ×50) from untreated control and control and bcl-2 transgenic mice 90 min after injection.