Figure 1.

The chimeric AML/ETO fusion protein is directed to the nuclear matrix in situ in the absence of the AML1B NMTS. Immunofluorescence localization of AML1B (Top), AML/ETO (Middle), and ETO (Bottom) proteins expressed in Saos-2 cells was examined in whole-cell (Left) and in situ nuclear-matrix (NMIF, Right) preparations by using specific antibodies to detect the epitope-tagged proteins and visualized by using a FITC secondary antibody. 4′,6-diamindino-2-phenylindole (DAPI) staining was used to evaluate DNA content and is shown (Inset) for each image. (Bar = 10 μm.)