Juvenile hormone (JH) reactivity in response to vitellogenin RNA interference (RNAi) in 7-day-old workers of selected pollen-hoarding strains. A, Mean vitellogenin messenger RNA level in noninjected controls, controls injected with double-stranded RNA derived from green florescent protein (GFP), and vitellogenin gene knockdowns in two replicate cage setups (upper and lower panels; n = 25 21, and 22 for vitellogenin RNAi, GFP control, and noninjected control, respectively). B, Mean JH response to vitellogenin downregulation for each replicate in the corresponding panel of A (n = 63 38, and 40 for vitellogenin RNAi, GFP control, noninjected control, respectively). Error bars indicate standard errors; lowercase letters indicate significant difference between strains (Fisher least significant difference post hoc tests: P < .05).