Structural analysis of BIT translocation between chromosome VIII and XV. (a) Schematic representation of the translocation between chromosomes VIII and XV with the location of the strategic loci tested. The interruption of the chromosome indicates the putative location of the DSB. (b) Southern hybridization of SanI, D10 Big, and D10 Small translocants with a DNA probe for the genes indicated below each panel. In the KANR panel, lanes 1 and 4, the positions of the bands corresponding to chromosome XV and VIII are shown, respectively, whereas lanes 2 and 3 show the bands corresponding to the translocated XV/VIII chromosome in the two strains D10 Big and Small. The positions of these four bands serve as a reference for all panels.