Fig. 2.

Ahr deficiency reduces IL-17 production in naïve T cells. (A) Purified naïve T cells were stimulated with anti-CD3/CD28 beads in the presence of IL-6 or TGF-β, either alone or combined. Supernatants were collected 4 days after stimulation, and IL-17 production was measured by means of ELISA. Data show means ± SE of three independent experiments. (B and C) Dot plots show intracellular staining for IFN-γ and IL-17. (B) Isolated naïve T cells from Ahr WT, He, and KO splenocytes were cultured with anti-CD3/CD28 beads and the indicated cytokines for 4 days. (C) Naïve T cells isolated from Ahr WT and KO mice were stimulated with anti-CD3/CD28 beads and TGF-β plus IL-6 in the presence or absence of TCDD or FICZ for 3 days. (D) Naïve T cells isolated from Ahr WT and KO mice were stimulated with anti-CD3/CD28 beads and the indicated cytokines for 2 days. Total RNA and cDNA were prepared as described in Methods. RORγ and RORα induction was examined by using RT-PCR. (B–D) These results are representative of three independent experiments.