Fig. 3.

Different pattern of IL-17 production between CD4⁺CD62L⁻ and CD4⁺CD62L⁺ cells. CD4⁺CD62L⁻ and CD4⁺CD62L⁺ cells isolated from WT mice were stimulated with anti-CD3/CD28 beads and TGF-β plus IL-6. (A) Three days after stimulation, cells were re-stimulated with PMA and ionomycin for 5 h and with GolgiStop (final 2 h), and then subjected to intracellular cytokine staining. Dot plots show intracellular staining for IFN-γ and IL-17. (B) Two days after stimulation, total RNA and cDNA were prepared as described in Methods. RORγ and Ahr induction was examined by using RT-PCR. These results are representative of three independent experiments.