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. 2008 Jul 10;105(28):9610–9615. doi: 10.1073/pnas.0803189105

Fig. 3.

Fig. 3.

Localization of homology models for the N-terminal region of the RyR1 in the 3D map. (A) Comparative models for the N-terminal region of RyR1 and corresponding structural templates. Model 1 (residues Q12–S207) is shown with cyan ribbon; model 2 is composed of two parts, shown with yellow (residues G216–T407) and red (residues A408–Y565) ribbons. Two parts of model 2 were built and refined independently to obtain better fits to the cryo-EM map. Final models were obtained through refinement of initial models using Moulder-EM (22). Dashed lines in template structures indicate regions that were not resolved in original x-ray structures (19). (B) The RyR1 monomer is shown in a side view along with homology models docked to the clamp region. Subregions are numbered. (C) Refined homology models are shown fitted within the density region segmented from the 9.6-Å map. The segmented density region is displayed in the orientation derived from its position in B by rotation to ∼90° around the axis as shown. SSEHunter-identified α-helices and β-sheets are shown with purple cylinders and yellow solid densities, respectively. N- and C-terminal residues in both models are indicated.