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. 2008 Jul 7;105(28):9751–9756. doi: 10.1073/pnas.0710413105

Fig. 4.

Fig. 4.

Differentiation precedes apoptosis in combination-treated neuroblastoma cells. (A) BE (2)-C cells were treated with either vehicle (veh) or the combination of 5 μM ATRA (A) and 1 mM VPA (V) for 6, 24, or 72 h and the effects on the 14-gene differentiation signature were evaluated. *, statistical significance in a pairwise t test comparing vehicle with drug treatment at each time point. Duplicate biological replicates and 16 technical replicates for each time point and condition were evaluated. BE (2)-C cells were treated in triplicate as above and the effects on early apoptosis (annexin V-FITC positive and PI negative) (B) and late apoptosis (annexin V-FITC positive and PI positive) (C) were evaluated. *, statistical significance in a pairwise t test comparing vehicle with drug treatment at each time point.