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. 2008 Jul 9;105(28):9727–9732. doi: 10.1073/pnas.0803644105

Fig. 3.

Fig. 3.

Ro52/SSA-reactive and La/SSB-reactive SLE IgG memory B cell antibodies. (A) LIA with 13 SLE-associated autoantigens (SmB, SmD, RNP-70k, RNP-A, RNP-C, Ro52/SSA, Ro60/SSA, La/SSB, Cenp-B, Topo-1/Scl-70, Jo-1/HRS, Ribosomal P, Histones) identified four La/SSB-reactive (29, 162, 264, and 276) and two Ro52/SSA-reactive (107 and 128) antibodies among 200 tested IgG memory B cell antibodies from SLE patients and 84 tested IgG memory B cell antibodies from HC. Anti-Ro52/SSB and anti-La/SSB antibodies were all from SLE175. LIA result obtained with serum from the same patient is shown for comparison. HEp-2 cell IFA staining patterns of the same antibodies are shown. (B) Germ-line versions of the two anti-Ro52/SSA and four anti-La/SSB reactive IgG memory B cell antibodies from SLE175 were tested by LIA and IFA as in A. (C) HEp-2 ELISA results of recombinant mutated (filled symbols) anti-La/SSB (29, 162, 264, 276; Left) and anti-Ro52/SSA (107, 128; Right) antibodies and their germ-line counterparts (open symbols). Red lines in the ELISA graphs represent low positive control serum, dotted lines represent ED38 (61), and horizontal lines show cut-off OD405 for positive reactivity.