Table 1.
Genes analyzed by quantitative PCR
| AGI | BL | IAA | Annotation | Greater | Equal | Longer | TGTCTC | TGTCT | TTGTCT |
|---|---|---|---|---|---|---|---|---|---|
| At4 g08950 | 3.2 | NC | Phi-1-like | + | ND | 1 | 1 | 0 | |
| At3 g45970 | 3.9 | NC | AtEXPL1 | + | ND | 1 | 1 | 1 | |
| At1 g62440 | 2.9 | NC | LRX2 | + | + | 4 | 5 | 2 | |
| At1 g29490 | 4.7 | 24.9 | SAUR-68 | + | + | 0 | 5 | 1 | |
| At4 g30290 | 3.7 | 10.9 | AtXTH19 | + | + | 1 | 3 | 3 | |
| At1 g52830 | 3.6 | 3.4 | IAA6 | + | + | 1 | 3 | 1 | |
| At3 g55840 | 4.1 | 2.6 | Hs1pro-1-like | + | ND | 0 | 1 | 0 | |
| At2 g23170 | NC | 147.8 | GH3.3 | + | ND | 1 | 3 | 0 | |
| At4 g32280 | NC | 27.0 | IAA29 | + | ND | 0 | 0 | 0 | |
| At4 g14560 | NC | 20.6 | IAA1 | + | ND | 0 | 2 | 1 | |
| At5 g02760 | NC | 11.5 | PP2C | + | + | 0 | 2 | 1 | |
| At5 g47370 | NC | 10.0 | Homeobox-leucine zipper | + | ND | 0 | 0 | 0 |
Brassinolide (BL) and IAA columns show level of induction observed in global transcriptome experiments after 2.5- or 3-h hormone treatments, respectively (6). Expression in the presence of both hormones was characterized as either greater than either hormone alone or equal to the level achieved under a single hormone treatment. Increased duration of expression was assessed for genes where single hormone treatments returned to mock levels by the 6-h time point. The number of times each variant of the ARF-binding site appears in the 1,000 bp upstream of the translation start site of each gene is listed. NC, no change; ND, could not be determined.