Fig. 6.
Mutation of the CREB binding site blocked RA-mediated induction of caspase-8 transcription. The sequence of the 105 bp RA-responsive region (C8frag04) is shown in the upper right panel. The putative transcription factor binding sites for CREB and c-Myb are underlined. Constructs containing single mutations in CREB or c-Myb binding sites and a double mutant were generated and used for transient transfection (right panel, lower). The relative luciferase activity was determined (left panel). The values represent the means ± SD from at least 3 independent experiments.
