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. Author manuscript; available in PMC: 2009 Apr 30.
Published in final edited form as: J Am Chem Soc. 2008 Apr 9;130(17):5681–5688. doi: 10.1021/ja0763201

Figure 4.

Figure 4

The distribution of structure in the transition state ensembles of C-terminally stabilized ankyrin repeat constructs, and the shift in the energy landscape from adding consensus repeats. Left: the transition state ensemble of the full length Notch ankyrin domain (A) is compared with that of Nank1-5 (B), Nank1-5C1 (C), and Nank1-5C2 (D). Repeats with high Φ-values are shaded green; those with zero Φ-values are shaded white. Constructs for which Φ-values could not be determined, Nank1-5 (AG2) and Nank1-5 (AG3), are colored grey. All Φ-values are mapped onto the crystal structure of Nank1-7Δ. Ribbon diagrams were generated using MacPymol.45. Right: energy landscapes, showing free energy (vertical direction) as a function of the number of folded repeats (back to front for denatured to native state, respectively) and the localization of structure (left to right for N- and C-terminally localized structure, respectively). Landscape tiers are shaded corresponding to Φ-values as in the ribbon diagrams.