Table 2. Fitted unfolding and refolding kinetic parameters for variants that contain alanine to glycine substitutions in Nank1-5, Nank1-5C₁, and Nank1-5C₂^a.

	kf,H2O (sec-1)	mf (M-1)	ku,H2O (sec-1)	mu (M-1)	b (M-2)	c (M-3)	${\Phi }_f^{H_{2}O}$
Nank1-7Δb	0.48	-0.22	0.00	1.702	-0.17
Nank1-5	0.69	-0.76	0.01	0.40
Nank1-5 (AG1)	0.65	-0.72	0.01	0.41			0c
Nank1-5 (AG4)	0.07	-1.76	0.07	0.61			0.60
Nank1-5 (AG5)	0.13	-1.52	0.04	0.62			0.69
Nank1-5C1	5.92	-0.24	1.68×10-5	1.27	-0.09
Nank1-5C1 (AG1)	5.92	-0.20	1.78×10-5	1.28	-0.09		0c
Nank1-5C1 (AG2)	6.97	-0.26	3.54×10-3	1.14	-0.08		-0.03
Nank1-5C1 (AG3)	8.43	-0.43	3.64×10-3	0.86	-0.08		-0.06
Nank1-5C1 (AG4)	4.53	-0.01	3.70×10-4	1.65	-0.33	0.02	0.09
Nank1-5C1 (AG5)	0.44	-0.32	1.59×10-6	1.72	-0.12		0.87
Nank1-5C1 (AGC1)	0.89	-0.03	4.04×10-5	1.62	-0.22	0.02	0.6
Nank1-5C2	34.7	-0.11	1.16×10-6	1.25	-0.07
Nank1-5C2 (AG1)	37.2	-0.14	1.71×10-6	1.21	0.07		0c
Nank1-5C2 (AG2)	18.4	-0.02	7.01×10-7	1.47	-0.07		0.08
Nank1-5C2 (AG3)	35.3	-0.06	1.83×10-5	1.27	-0.09		0.00
Nank1-5C2 (AG4)	51.5	-0.20	1.36×10-4	0.98	-0.07		-0.06
Nank1-5C2 (AG5)	32.3	-0.03	4.77×10-7	1.60	-0.11		0.02
Nank1-5C2 (AGC1)	1.91	0.10	5.02×10-10	2.24	-0.14		1.05
Nank1-5C2 (AGC2)	11.1	-0.21	5.70×10-7	1.47	-0.09		0.40

^aParameters were estimated by fitting equation (2) to the urea dependencies of the rate constants of the major refolding and unfolding phases.

^bData taken from reference (22).

^cΦ-values of zero were assumed for substitutions in the first repeat, because folding rates are not affected by the substitution. Conditions: 25 mM Tris•HCl (pH 8.0), 150 mM NaCl, 20 °C.