Figure 7. Effect of KChIP expression on the levels of NCX2 and NCX3 Na⁺/Ca²⁺ exchangers.

(A) PC12 cells were transfected to express KChIP–ECFP constructs as indicated, fixed and stained with anti-NCX2 or NCX3. Microscope fields were examined that contained both transfected cells and non-transfected cells as seen in the ECFP images. Cells transfected with KChIP3–ECFP showing lower NCX3 immunofluorescence levels are indicated by arrows. The scale bar represents 10 μm. (B) Fluorescence levels from transfected and adjacent control non-transfected cells were determined (n=12) and the values for transfected cells were expressed as a percentage of the appropriate controls. *P<0.001 compared with non-transfected control cells.