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. 2008 Jun 26;413(Pt 2):323–332. doi: 10.1042/BJ20080321

Figure 5. Effect of CAtr on HNE-induced ANT activation.

Figure 5

(A) Rat liver mitochondria incubated in KPHEB plus 5 μM rotenone, 1 μg/ml oligomycin and 80 ng/ml nigericin ±35 μM HNE were energized with 4 mM succinate. Where indicated, 2.5 μM CAtr was added at the start of the experiment (CAtr before En), or 1 min, 3 min or 5 min after succinate addition (CAtr after En). After 5 min of energization (En), proton leak kinetics were determined by malonate titration (up to 2.3 mM). Values are means±S.E.M. of four independent experiments. (B) Proton leak rates through ANT at the highest common potential. Data are calculated as each condition minus its ‘plus CAtr (and before HNE)’ condition for each independent experiment and subsequently averaged to give means±S.E.M. of four independent experiments. For HNE data, **P<0.01 compared with #.