Fig. 3.

Treatment with anti-PrP shRNA expressing lentivirus prolongs survival in mice with established prion disease. Mice were infected with RML prions at 1 week of age and were treated with bilateral hippocampal injections of either LV-MW1 (n = 22) or LV-Empty (n = 18) at 8 wpi or with no virus (n = 18). RML-infected mice treated with no virus or with LV-Empty died within 91 and 101 days postinfection (dpi), respectively; mice treated with LV-MW1 survived longer, living up to 129 dpi. (P < 0.0001 Student's t test, two tails, compared with both LV-Empty-treated and untreated mice).