Table 1.

List of genes induced ≥2-fold by HFD feeding in wild-type mice and reduced by at least 25% in HFD-fed Alb::GC transgenic mice compared to high fat diet (HFD)-fed wildtype (WT). "Induction" is the ratio of the hybridization signals in LFD-fed versus HFD-fed mice. "% of GC in WT" is defined as the percentage of the signal of HFD-fed Alb::GC in HFD-fed WT mice. The mean ± the average deviation of the expression level (see methods) in WT and Alb::GC from two independent mice is shown. (A) A list of genes encoding transcription factors that satisfy the above criteria but are also induced ≥ 2-fold in AP20187-injected (0.2µg/gm body weight) Ttr::Fv2E-PERK high expressing (line #30) transgenic mice. (B) A list of genes involved in carbohydrate, lipid and amino acid metabolism that satisfy the above criteria.

A
			Expression level				HFD induction (fold)
		Entrez	WT		Alb::GC		WT	Alb::GC	% of GC in WT
Name	Description	Gene	LFD	HFD	LFD	HFD	HFD/LFD	HFD/LFD	HFD
Transcription factor
Pparg	peroxisome proliferator activated receptor gamma	19016	0.97 ± 0.28	4.05 ± 0.04	0.68 ± 0.71	0.25 ± 0.11	4.18 ± 1.03	0.37 ± 0.13	6 ± 2
Crsp6	cofactor required for Sp1 transcriptional activation, subunit 6	234959	0.69 ± 0.13	2.29 ± 0.51	1.02 ± 0.33	0.65 ± 0.22	3.30 ± 0.09	0.63 ± 0.01	28 ± 3
Jarid1d	jumonji, AT rich interactive domain 1D (Rbp2 like)	20592	0.49 ± 0.14	1.38 ± 1.14	1.74 ± 3.07	0.45 ± 0.35	2.84 ± 1.01	0.26 ± 0.12	32 ± 1
Klf13	Kruppel-like factor 13	50794	0.65 ± 0.27	4.20 ± 0.19	0.87 ± 0.16	1.41 ± 0.63	6.44 ± 1.95	1.61 ± 0.33	33 ± 11
Ppara	peroxisome proliferator activated receptor alpha	19013	0.83 ± 0.13	5.41 ± 0.29	0.61 ± 0.26	1.82 ± 1.35	6.51 ± 0.57	2.99 ± 0.59	34 ± 18
Sox-4	SRY-box containing gene 4	20677	0.86 ± 0.45	2.93 ± 0.60	0.67 ± 0.02	1.02 ± 0.53	3.41 ± 0.81	1.54 ± 0.61	35 ± 8
Nfic	nuclear factor I/C	18029	0.47 ± 0.05	3.80 ± 0.38	0.59 ± 0.03	1.67 ± 0.35	8.04 ± 0.01	2.81 ± 0.40	44 ± 4
Cphx	cytoplasmic polyadenylated homeobox	105594	0.28 ± 0.37	1.55 ± 0.62	0.13 ± 0.04	0.69 ± 0.22	5.49 ± 2.81	5.24 ± 0.12	44 ± 3
Akna	AT-hook transcription factor	100182	0.39 ± 0.24	1.69 ± 0.48	0.60 ± 0.14	0.79 ± 0.25	4.33 ± 1.79	1.31 ± 0.35	47 ± 6
Mxd3	Max dimerization protein 3	17121	0.29 ± 0.06	1.10 ± 0.09	0.54 ± 0.23	0.56 ± 0.52	3.84 ± 0.44	1.04 ± 0.32	51 ± 31
Mef2a	myocyte enhancer factor 2A	17258	0.27 ± 0.02	1.73 ± 0.19	0.51 ± 0.81	0.96 ± 0.32	6.39 ± 0.29	1.88 ± 1.33	55 ± 10
Klf3	Kruppel-like factor 3 (basic)	16599	0.74 ± 0.04	1.74 ± 0.08	0.93 ± 0.04	1.04 ± 0.17	2.37 ± 0.01	1.12 ± 0.12	60 ± 6
Nfkb2	NF-kappaB2/p100	18034	0.27 ± 0.03	3.27 ± 0.10	0.32 ± 0.00	1.98 ± 0.36	11.93 ± 0.81	6.27 ± 0.97	61 ± 8
Bach2	BTB and CNC homology 2	12014	0.52 ± 0.62	1.90 ± 0.18	0.50 ± 0.11	1.16 ± 0.39	3.66 ± 2.73	2.29 ± 0.23	61 ± 12
Zkscan1	zinc finger with KRAB and SCAN domains 1	74570	0.73 ± 0.14	1.60 ± 0.05	0.98 ± 0.04	1.11 ± 0.14	2.20 ± 0.33	1.13 ± 0.09	69 ± 6

B
			Expression level				HFD induction (fold)
		Entrez	WT		Alb::GC		WT	Alb::GC	% of GC in WT
Name	Description	Gene	LFD	HFD	LFD	HFD	HFD/LFD	HFD/LFD	HFD
Carbonhydrate metabolism
Pdha2	pyruvate dehydrogenase E1 alpha 2	18598	0.16 ± 0.02	0.37 ± 0.26	0.23 ± 0.14	0.14 ± 0.06	2.26 ± 1.01	0.61 ± 0.07	39 ± 7
Pdk3	pyruvate dehydrogenase kinase, isoenzyme 3	236900	0.33 ± 0.02	0.87 ± 0.09	0.38 ± 0.05	0.37 ± 0.06	2.61 ± 0.14	0.98 ± 0.03	42 ± 3
Mdh2	malate dehydrogenase 2, NAD (mitochondrial)	17448	0.75 ± 0.18	1.91 ± 0.10	0.64 ± 0.04	1.11 ± 0.04	2.54 ± 0.42	1.72 ± 0.04	58 ± 1
Agl	amylo-1,6-glucosidase, 4-alpha-glucanotransferase	77559	0.85 ± 0.13	1.85 ± 0.07	0.71 ± 0.04	1.09 ± 0.09	2.17 ± 0.23	1.54 ± 0.03	59 ± 2
Ppp2r5e	protein phosphatase 2, regulatory subunit B (B56), epsilon	26932	0.43 ± 0.03	2.57 ± 0.19	0.45 ± 0.35	1.52 ± 0.40	6.00 ± 0.02	3.37 ± 1.17	59 ± 10
Ppp1r1a	protein phosphatase 1, regulatory (inhibitor) subunit 1A	58200	0.60 ± 0.11	1.39 ± 0.04	0.79 ± 0.10	0.84 ± 0.20	2.33 ± 0.32	1.07 ± 0.09	61 ± 11
Gyg	glycogenin	27357	0.68 ± 0.09	1.99 ± 0.12	0.31 ± 0.61	1.26 ± 0.20	2.93 ± 0.18	4.11 ± 4.48	63 ± 6
Pcx	pyruvate carboxylase	18563	0.72 ± 0.07	1.90 ± 0.08	0.72 ± 0.11	1.19 ± 0.08	2.64 ± 0.17	1.66 ± 0.20	63 ± 2
Pklr	pyruvate kinase liver and red blood cell	18770	0.66 ± 0.09	1.67 ± 0.10	0.99 ± 0.15	1.11 ± 0.06	2.53 ± 0.25	1.12 ± 0.07	66 ± 3
Gbe1	glucan (1,4-alpha-), branching enzyme 1	74185	0.60 ± 0.01	2.07 ± 0.17	0.71 ± 0.01	1.40 ± 0.14	3.43 ± 0.22	1.96 ± 0.15	68 ± 1
Suclg2	succinate-Coenzyme A ligase, GDP-forming, beta subunit	20917	0.73 ± 0.02	1.59 ± 0.03	0.84 ± 0.06	1.13 ± 0.04	2.17 ± 0.02	1.34 ± 0.05	71 ± 1
Lipid metabolism
Scd1	stearoyl-Coenzyme A desaturase 1	20249	0.81 ± 0.19	3.43 ± 0.11	1.18 ± 0.25	0.90 ± 0.10	4.24 ± 0.77	0.76 ± 0.06	26 ± 2
Fasn	fatty acid synthase	14104	0.85 ± 0.25	2.26 ± 0.09	0.98 ± 0.08	0.88 ± 0.06	2.66 ± 0.58	0.90 ± 0.01	39 ± 1
Gpd2	glycerol phosphate dehydrogenase 2, mitochondrial	14571	0.86 ± 0.29	1.86 ± 0.05	0.89 ± 0.03	0.84 ± 0.24	2.17 ± 0.58	0.95 ± 0.21	45 ± 10
Lpl	lipoprotein lipase	16956	0.66 ± 0.15	1.96 ± 0.68	0.83 ± 0.36	0.93 ± 0.48	2.95 ± 0.27	1.13 ± 0.06	48 ± 6
Olah	oleoyl-ACP hydrolase	99035	0.12 ± 0.00	0.34 ± 0.07	0.12 ± 0.01	0.17 ± 0.05	2.82 ± 0.47	1.43 ± 0.27	50 ± 4
Acaca	acetyl-Coenzyme A carboxylase alpha	107476	0.62 ± 0.43	1.82 ± 0.30	0.71 ± 0.02	1.11 ± 0.19	2.94 ± 1.12	1.58 ± 0.20	61 ± 0
Gyk	glycerol kinase	14933	0.53 ± 0.04	1.84 ± 0.06	0.69 ± 0.16	1.16 ± 0.00	3.46 ± 0.16	1.68 ± 0.35	63 ± 2
Hadh	hydroxyacyl-Coenzyme A dehydrogenase	15107	0.32 ± 0.16	2.78 ± 0.02	0.25 ± 0.02	1.87 ± 0.44	8.57 ± 3.53	7.45 ± 0.94	67 ± 14
Translation, amino acid import and metabolism
Eif3s6	eukaryotic translation initiation factor 3, subunit 6	16341	0.42 ± 0.11	1.03 ± 0.18	0.81 ± 0.92	0.33 ± 0.11	2.43 ± 0.14	0.41 ± 0.21	32 ± 4
Aoc3	amine oxidase, copper containing 3	11754	0.21 ± 0.04	0.67 ± 1.23	0.55 ± 1.35	0.19 ± 0.05	3.17 ± 3.17	0.35 ± 0.42	29 ± 26
Pgd	phosphogluconate dehydrogenase	110208	0.91 ± 0.18	2.04 ± 0.05	1.00 ± 0.11	0.82 ± 0.02	2.24 ± 0.35	0.82 ± 0.06	40 ± 0
Rps18	ribosomal protein S18	20084	0.61 ± 0.11	2.39 ± 2.01	1.03 ± 0.09	0.96 ± 0.08	3.91 ± 1.77	0.93 ± 0.01	40 ± 22
RPL39L	ribosomal protein L39-like	68172	0.50 ± 0.20	1.32 ± 0.71	0.12 ± 0.03	0.75 ± 0.03	2.65 ± 0.26	6.33 ± 1.31	57 ± 23
Slc12a1	solute carrier family 12, member 1	20495	0.16 ± 0.10	1.80 ± 0.07	0.57 ± 0.84	1.25 ± 1.38	11.52 ± 5.41	2.22 ± 0.37	70 ± 53
Tph1	tryptophan hydroxylase 1	21990	0.44 ± 0.14	1.40 ± 0.34	1.25 ± 0.55	0.99 ± 0.43	3.22 ± 0.23	0.79 ± 0.00	71 ± 10