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. Author manuscript; available in PMC: 2009 Apr 8.
Published in final edited form as: Circulation. 2008 Mar 24;117(14):1810–1819. doi: 10.1161/CIRCULATIONAHA.107.725481

Figure 5. Effects of FA treatment on ROS generation.

Figure 5

A] Lucigenin-enhanced superoxide detection shows marked reduction of ischemia and IR-induced superoxide with FA pre-treatment. B] O2- formation in control-vehicle treated IR hearts was markedly suppressed by acute addition of BH4, whereas this was blunted by nearly half in myocardial extracts obtained from FA-pre-treated hearts. C-D] DHE and E] DCF stained myocardium reveal increased ROS generation in ischemic or IR myocardium that was substantially reduced in heart receiving FA pre-treatment. F] Direct antioxidant effects of FA compared with Tempol. Superoxide was generated by xanthine/xanthine oxidase in vitro, and measured by lucigenin-enhanced chemiluminescence at varying added concentrations of either FA or tempol.