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. 1999 Dec 21;96(26):14919–14924. doi: 10.1073/pnas.96.26.14919

Figure 6.

Figure 6

Competitive inhibition of the in vitro association. The assay was performed by using 20 μg of IPM from nontransfected cells and/or IDM from mycGlut4 cells in the presence of 3-fold excess (0.8 mg/ml) of donor membrane from nontransfected adipocytes or BSA (A). (B) Assays were performed with IDM from mycGlut4 cells and IPM from nontransfected cells in the presence of the indicated concentration of GST-syntaxin 4. The total added protein concentration was normalized by the addition of GST protein. The quantified values of association as measured by mycGlut4 immunoreactivity are expressed as a percentage of the value without GST-syntaxin 4 (n = 4).