Weight of gastric contents (a) and serum (b) and gastric (c) leptin levels in 4-day-old rats treated with an oral dose of leptin or vehicle at time 0 and 4 h later. Five different litters were used to perform the experiment and pups from each litter were randomly assigned into the different groups. The values from each litter are expressed as a percentage of its time 0 group. Within each graph, bars not sharing a common letter (a, b, c,) are significantly different. Adapted from [12] (Copyright 2005, The Endocrine Society)