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Purification of MAP65. The carrot MAP fraction was purified by sucrose density centrifugation. The silver-stained gel of the fractions (28–5) shows separation of MAP65 from MAP120. Vertical axis, molecular weight markers; horizontal axis, key fractions. (Inset) Pooled MAP65 fraction showing the predominant 62-kDa member of the MAP65 triplet; MAP68 is shown as the thin band above.
