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. 1972 Jun;110(3):831–842. doi: 10.1128/jb.110.3.831-842.1972

Conjugational Complementation Analysis of Transfer-Deficient Mutants of Flac in Escherichia coli

Mark Achtman a,1, Neil Willetts a, Alvin J Clark a
PMCID: PMC247500  PMID: 4555412

Abstract

A series of 102 transfer-deficient (tra−) mutants of Flac (84 of which have been previously described) were classified as carrying frameshift, amber, ochre, UGA, and nonsuppressible mutations. Techniques were evolved for using cultures of F prime strains as efficient recipients in matings, for measuring the number of (F′/F′) heterozygote cells in transient populations, and for measuring complementation between different Flac tra− mutants. These techniques were used to define nine tra cistrons and to assign most of the tra− mutations to one or other of these.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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