Figure 3.

Rapid aneuploidy in TGF-β1−/− keratinocytes after transduction with v-ras^Ha. Percentages of metaphases with specific chromosome numbers in TGF-β1+/+ and TGF-β1−/− keratinocytes at the indicated times after infection with the v-ras^Ha retrovirus. Each histogram represents the sum of three independent chromosome harvests. Between 50 and 100 metaphases were counted for each genotype at a specific time point, and the percentage of total mitoses with a specific chromosome number was plotted. Metaphase spreads with an extremely high chromosome number were excluded from the histogram for uniformity. (A–C) TGF-β1+/+ keratinocytes. (D–F) TGF-β1−/− keratinocytes. Results are for 4 days (A and D), 8 days (B and E), and 15 days (C and F) after infection of TGF-β1+/+ and TGF-β1−/− keratinocytes with v-ras^Ha.