Figure 5.

Binding of wild-type and mutant TRP-C7 to IP3R-F2 and partial interference of agonist and TG-induced Ca²⁺ entry by TRP3-C7 but not by fragments that do not interact with IP3R. (A) Binding of TRP3 fragments to IP3R-F2. (B) Summary of IP3R-F2 binding to C-terminal fragments of hTRP3. (C) Effect of EBFP and EBFP-TRP fusions on Ca²⁺ entry stimulated by TG (C2) or by AVP in cells cotransfected with the Gq-coupled V1a receptor (C1). C7, hTRP3[742–795], C7^mut3, hTRP3[742–795] with K780D/R781E/K784D/R785E. The strategy and methods described in ref. 19 were used to determine Ca²⁺ transients. Points are means ± SEM of the number of cells shown analyzed in three to four independent transfections. Insets, Significance of differences between [Ca²⁺]_i curves; P value, Student's t test of the null hypothesis.