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. 2008 Aug 1;4(8):e1000117. doi: 10.1371/journal.ppat.1000117

Figure 4. Steady State Localization of Leg-GFP Hybrids and Vacuole Defects.

Figure 4

(A) Yeast strains expressing the LegC2-GFP, LegC3-GFP, LegC7-GFP hybrid proteins and GFP alone were harvested and pulse-chased with N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenylhexatrienyl)-pyridinium dibromide (FM4-64) for vacuole visualization. Cells were viewed using epifluorescence microscopy. The images shown are representative of the overall population of cells expressing the Leg-GFP hybrids. (B) Whole cell lysate immunoblot using a rabbit polyclonal antibody against GFP showing that all the GFP Leg fusion proteins are expressed under galactose induction, but not when grown in fructose. The asterisk (*) is placed to emphasize that ten times more LegC7-GFP sample had to be loaded for visualization.