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. Author manuscript; available in PMC: 2008 Jul 21.
Published in final edited form as: J Mol Biol. 2007 Mar 30;369(3):812–828. doi: 10.1016/j.jmb.2007.03.065

Figure 1.

Figure 1

Nanospray-FTICR mass spectra of samples containing 30 μM NC (●) and 10 μM of either a) wild-type SL1B (◆), or b) SL1B-A272G (▽) in 150 mM ammonium acetate (pH 7.0) at room temperature. The signal corresponding to the −7 ion of the NC-SL1B complex is enlarged in the inset to show how the charge state of each species is unambiguously determined from the respective isotopic spacing. The corresponding molecular mass is then readily obtained from the charge state and the position of the peak on the m/z scale. Note that a maximum 2:2 protein to RNA ratio was observed for the KL assembly of wild-type SL1B, whereas a single base substitution in the junction region allowed for the binding of a third equivalent of NC.