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. Author manuscript; available in PMC: 2008 Jul 21.
Published in final edited form as: J Mol Biol. 2007 Mar 30;369(3):812–828. doi: 10.1016/j.jmb.2007.03.065

Figure 2.

Figure 2

Product ion spectra obtained by submitting a) the KL-obligated dimer (■□) and b) the ED-obligated dimer (◀▷) to mild SORI-CID (see Materials and Methods). Panel c) was obtained by submitting the ED-obligated dimer to harsher activation conditions. Boxed symbols identify each precursor ion. Note that the KL dimer readily dissociated into its monomeric components, whereas the ED conformer remained intact under identical activation conditions. At more energetic regimes, the latter provided typical d-H2O and y series, which were produced by covalent fragmentation of the RNA backbone.