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. 2008 Jun 16;36(12):4172–4180. doi: 10.1093/nar/gkn360

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Design and optimization of self-cleavage conditions for an active-site tethered ES conjugate. (A) Illustration of pt^Tyr-S₃ (and S₅)-M RPR in which ptRNA^Tyr is attached to L15 of Mja RPR with either a 3- or 5-nt spacer. The C and S domains are demarcated to indicate what was deleted in pt^Tyr-S₃-ΔS M RPR. (B) Titration of monovalent and divalent cations to identify the optimal conditions for maximal self-cleavage of 5′ labeled pt^Tyr-S₅-M RPR (see text for details). R and L indicate RPR cis conjugate and the 5′ leader, respectively.