Figure 2.

Both 3′ protected and unprotected small RNAs are bound by HC-Pro and p19. RNA for northern blots were isolated from IPs performed on extracts made from systemic leaves of TEV (A) and CIRV (C) infected plants. In the case of mock IP, no antibody was used. For TEV, 10% of the input and the eluate of the IP were loaded. In the case of CIRV, 10% of input and 100% of the IP eluate were loaded. Hybridizations were performed with the indicated probes. U6 RNA serves as control for IPs, 10 pmol of synthetic GFP RNA oligo was used as an internal control for β-elimination. Western blots were loaded with protein extracts of inputs and eluates of HC-Pro (B) and p19 (D) IPs.