Figure 4.

Co-expression of silencing suppressors with miRNA precursor. RNA samples for northern blots were isolated from agroinfiltrated N. benthamiana leaves with the designated constructs. Two sets of membranes containing the same samples were used. The membranewhich was used for the miR171a* hybridization resulting in no signal, was used later for miR171c* and miR171c hybridizations resulting in strong and specific signals. Hybridizations were performed with the indicated probes. U6 RNA serves as loading control. The 10 pmol of synthetic GFP RNA oligo was used as an internal control for β-elimination.