Figure 3.

Loss of Ku80 leads to error-prone end-joining (A) Sequences of NHEJ repair junctions based on pEJSSA obtained from CHO K1, xrs5 and xrs5 cells complemented with hKu80. For the parental and the cleaved sequences, both strands are shown. For the repair products, only the sense strand is displayed. I-SceI recognition sites are depicted in bold. The artificial start codon is underlined in the parental sequence. Del indicates the net loss or gain of base pairs, whereby both single-stranded overhangs were regarded as 4-bp double-stranded DNA. tμh indicates the number of terminal microhomologies used for rejoining. (B) Distribution of the length of deletions generated upon NHEJ. (C) Mean deletion length. Differences between CHO K1 and xrs5 cells were statistically significant (Mann–Whitney, P = 0.03).