Figure 4.

DREFdsRNA treatment affects mRNA levels and promoter activities of osa and mor. (A) cDNAs were prepared from total RNA isolated from dsRNA-treated S2 cells and levels of DREF, osa and mor mRNAs were measured by quantitative RT-PCR. Fold differences against the amplification with no treatment are shown with standard deviations from three independent dsRNA treatments. β-tubulin was used as a negative control. (B) Cell extracts were analyzed by western blotting with antiDREF antibodies. (C) Promoter activities of osa and mor in dsRNA-treated cells. Luciferase activities relative to that of wild-type promoter in non-dsRNA-treated cells are shown with standard deviations from five independent transfections.