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. 2008 Jun 10;36(12):4128–4136. doi: 10.1093/nar/gkn334

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Summary of DNA–Top1 complex formation by (A) unmodified DNA substrate and (B–C) CrA-PdG adducted DNA substrate. Boxed nucleotide, site of adduct; open triangles, no cleavage; closed triangles, Top1 cleavage site. (A) In the unmodified substrate, Top1 cleavage complexes are trapped by CPT at site 13. Sites 17 and 18 are undetectable. (B) In the CrA-PdG adducted substrate, site 13 is suppressed and two cleavage sites are induced; site 17 is CPT-independent and site 18 is trapped by CPT. (C) Upon reduction by NaCNBH3, Top1 forms an indirect crosslink with the DNA via the CrA-PdG adduct and the formation of Top1 cleavage complexes is inhibited.