Table 3.
8-oxo-dG bypass fidelity by RB69 polymerases
| L+ | L− | F+ | F− | |
|---|---|---|---|---|
| Light blue plaques | 87.5% (1108) | 84.6% (1549) | 91% (1065) | 82.3% (1205) |
| Dark blue plaques | 10% (127) | 13.7% (251) | 8.2% (96) | 17.2% (252) |
| dATP:Go (%) | 17 | 23 | 14 | 29 |
| C:A ratio | 5.0 | 3.4 | 6.3 | 2.5 |
In parentheses are the numbers of plaques scored. A small percentage of plaques were colorless due to sequence changes within the chemically synthesized oligonucleotides and/or due to occasional polymerization errors located at positions other than at the nonsense codon.