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. 2008 May 24;36(12):e69. doi: 10.1093/nar/gkn331

Figure 2.

Figure 2.

Delivery of NeutrAvidin conjugates into single living cells. Cy5-labeled NeutrAvidins were delivered into NIH/3T3 cells either by (a) TAT-peptide, (b) SLO or (c) microporation. Images corresponding to TAT-peptide-based delivery and microporation were acquired 24 h following delivery. Images corresponding to delivery via SLO were acquired immediately after the permeabilized membranes were resealed, ∼1.5 h. Subsequent staining of the cells with LysoTracker was used to determine whether the pegylated NeutrAvidin was localized within lysosomes for each of the delivery methods, (d) TAT-peptide, (e) SLO and (f) microporation. The merged NeutrAvidin and lysosome images for (g) TAT peptide, (h) SLO, and (i) microporation show all colocalized fluorescent signals as yellow in color. The contrast in the yellow channel of the merged images was increased to more clearly distinguish areas of colocalization from the pure red and green signals.