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. 2008 May 30;36(12):3956–3968. doi: 10.1093/nar/gkn348

Figure 1.

Figure 1.

Thermotoga maritima HU promotes DNA bending. (A and B) DNA cyclization assay in the absence or presence of TmHU as indicated above the panels. Lane 1 shows 105 bp linear duplex DNA. Reactions in lanes 2–8 contained 105-bp DNA incubated with T4 DNA ligase, and reactions in lanes 10–16 additionally contained 50 fmol TmHU. Reactions in lanes 2 and 10 were incubated with T4 DNA ligase for 1 min, reactions in lanes 3 and 11 for 2 min, reactions in lanes 4 and 12 for 8 min, reactions in lanes 5 and 13 for 11 min, reactions in lanes 6 and 14 for 14 min, reactions in lanes 7 and 15 for 17 min and reactions in lanes 8 and 16 for 30 min. Reactions in lanes 9 and 17 were treated with Exonuclease III following incubation with ligase for 30 min. The original 105 bp DNA, dimeric ligation product, intramolecular cyclization product and linear oligomers are identified at the right.