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. 2008 May 30;36(12):3956–3968. doi: 10.1093/nar/gkn348

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — DNA compaction by TmHU is salt-dependent. Agarose gel electrophoresis of HBsu, TmHU at 50 mM NaCl (TmHU) or TmHU at 200 mM NaCl (TmHU + NaCl) binding to supercoiled (s/c; top row), linear (lin; middle row), or nicked (nick; bottom row) pUC18 DNA. Protein concentrations, identical for all rows, are indicated at the bottom. Reactions in lanes 1 contain no protein, with the position of the free DNA identified at the left. DNA is stained with EtBr after electrophoresis; DNA in reactions with higher concentrations of TmHU fails to stain.