Table 4.

Kinetic parameters of correct nucleotide incorporation into the 44CTL DNA template

DNA substrate (P/T)	dNTP	kp (s−1)	Kd (μM)	kp/Kd (μM−1 s−1)
21-mer/44CTL	dGTP	9.3 ± 0.2	(2.1 ± 0.2) × 102	4.5 × 10−2
22-mer/44CTL	dATP	4.1 ± 0.1	(3.8 ± 0.4) × 102	1.1 × 10−2
23-mer/44CTL	dCTP	10.8 ± 0.4	(1.2 ± 0.1) × 102	9.3 × 10−2
24-mer/44CTL	dCTP	4.5 ± 0.2	(7 ± 2) × 101	6.3 × 10−2
25-mer/44CTL	dATP	15.4 ± 0.8	(1.6 ± 0.3) × 102	9.7 × 10−2
26-mer/44CTL	dGTP	9.3 ± 0.2	(5.5 ± 0.4) × 101	1.7 × 10−1
27-mer/44CTL	dATP	5.8 ± 0.2	(4.0 ± 0.5) × 102	1.3 × 10−2
28-mer/44CTL	dGTP	15.0 ± 0.8	(6.1 ± 0.8) × 102	2.5 × 10−2
29-mer/44CTL	dGTP	5.2 ± 0.5	(9 ± 2) × 102	5.6 × 10−3
30-mer/44CTL	dCTP	14.5 ± 0.9	(1.7 ± 0.3) × 102	8.5 × 10−2
31-mer/44CTL	dTTP	15.7 ± 0.9	(2.0 ± 0.4) × 102	7.7 × 10−2
32-mer/44CTL	dATP	21 ± 1	(2.6 ± 0.4) × 102	8.0 × 10−2