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. 2008 May 30;36(12):e71. doi: 10.1093/nar/gkn287

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Sensitized emission measurements of double-labelled LPEI-polyplexes by CLSM. HeLa cells were incubated with fluo-p3NF-luc-3NF/rho-LPEI polyplexes in the presence of TNFα. Fluorescein (fluo), rhodamine (rho) and Draq5 were excited at 488, 543 and 633 nm, respectively. (A) Transmitted light images merged with recorded images for the fluorescence emission of fluo-p3NF-luc-3NF (in green), rho-LPEI (in red) and Draq5 (in blue) in a multi-tracking mode. (B–D) FRET ratio images calculated according to the method of Youvan (B), Xia (C) and Gordon (D). Sidebars: colour pixels scoring for FRET level from 0 (dark blue) to 50 (white). Each image corresponds to 512 × 512 pixels measuring 0.28 × 0.28 µm² each.