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. 2008 May 30;36(12):e71. doi: 10.1093/nar/gkn287

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — FRET imaging of double-labelled polyplexes in living cells. C2C12 (A–D) and HeLa (E–H) cells were transfected for 5 h with fluo-p3NF-luc-3NF/rho-LPEI (A, B and E, F) or fluo-p3NF-luc-3NF/rho-HIS (C, D and G, H). NFRET ratio images were calculated according to Xia's normalization. Fluo, rho and Draq5 were excited at 488, 543 and 633 nm, respectively. Sidebars: colour pixels scoring for FRET level from 0 (dark blue) to 50 (white). Each image corresponds to 512 × 512 pixels measuring 0.28 × 0.28 µm² each.