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. 2008 May 31;36(12):4009–4021. doi: 10.1093/nar/gkn353

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Expression of eukaryotic HMGB derivatives in E. coli. Western blotting with anti-myc epitope antibodies showing protein extracts from E. coli cells carrying HMGB expression plasmids. (A) Proteins expressed at lower levels. Lanes 1–4 correspond to proteins 1200 (myc/Nhp6A), 1299 (myc/Nhp6AΔ(2–12), 1327 (Nhp6A/myc) and 1328 (Nhp6AΔ(2–12)/myc), respectively. (B) Proteins expressed at higher levels. Lanes 1–3 correspond to proteins 1328 [Nhp6AΔ(2–12)/myc; repeated from panel A for comparison], 1492 (myc/hHMGB2A) and 1493 [myc/hHMGB2AΔ(84–92)], respectively.