Table 1. Sequencing success and efficacy for six coding and three non-coding regions.
| Region: | cox1 | 23S rDNA | rpoB | rpoC1 | rbcL | matK | trnH-psbA | atpF–atpH | psbK–psbI |
| Aligned sequence length (bp) | 656 | 363 | 590 | 487 | 607 | 946 † | 200–760 †† | 242–735 †† | 260–673 †† |
| Unaligned length; mean (range), including end gaps | 656 (-) | 362.7 (359–363) | 470.5 (429–481) | 487 (-) | 606.8 (588–607) | 735.3 (325–895) | 392.7 (142–699) | 545.5 (240–589) | 403.0 (172–629) |
| Position in Arabidopsis thaliana gene (length) ‡ | 42–697 (656) | 2091–2453 (363) | 1704–2175 (472) | 1895–2381 (487) | 27–633 (607) | 525–1309 (785) | |||
| No. of species successfully amplified and sequenced * | 69 | 90 | 87 | 89 | 92 | 84 | 92 | 88 | 79 |
| No. of samples successfully amplified and sequenced ** | 170 | 236 | 231 | 238 | 251 | 220 | 249 | 239 | 214 |
| % sequencing success *** | 72.0 | 100.0 | 92.0 | 94.8 | 100 | 87.6 | 99.2 | 95.2 | 85.3 |
| Total no. primer pairs used | 1 | 1 | 5 | 3 | 2 | 10 | 1 | 1 | 1 |
| Mean number of reads in contig per sample¶ | 2.00 | 2.00 | 2.27 | 2.48 | 2.27 | 2.96 | 2.83 | 2.44 | 2.67 |
| % of sequences that are <80% bidirectional § | 1.1 | 0 | 4.7 | 6.9 | 2.4 | 25.5 | 19.7 | 6.3 | 27.1 |
Sequences from the first seven regions were sought for 251 samples representing 92 species. Cox1 and 23S rDNA were attempted for 236 samples and 90 species. The sequence ranges used in the analysis are provided in reference to the complete plastid and mitochondrial genomes of Arabidopsis thaliana (Genbank accessions NC 000932, NC 001284).
Aligned across angiosperms and gymnosperms only.
Aligned across individual genera only.
Based on trimmed alignments for coding regions.
92 species attempted, except 23S rDNA and cox1 (90 species).
251 individuals attempted for all genes except 23S rDNA and cox1 (236 individuals);
Percentage sequencing success (i.e., number of individuals successfully sequenced/number of individuals attempted);
The number of reads represents the mean number of unidirectional sequences from successful amplifications that are required to establish a reliable sequence for each sample;
Sequences with less than 80% bidirectional coverage are primarily due to the presence of homopolymer runs.