Table 2. Number of species per genus resolved as monophyletic for each of nine candidate barcoding regions.
| Major clade | Genus | No. of species surveyed | Region | ||||||||
| cox1 | 23S rDNA | rpoB | rpoC1 | rbcL | matK | trnH-psbA | atpF–atpH | psbK–psbI | |||
| Angiosperms | Acer | 5 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 0 | 0 |
| Betula | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | M | 0 | |
| Cornus | 4 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 1 | 3 | |
| Erigeron | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| Eupatorium | 2 | 0 | 0 | 2 | 0 | 2 | 2 | 2 | 2 | 2 | |
| Lactuca | 2 | NA | NA | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |
| Plantago | 3 | 3 | 1 | 1 | 1 | 1 | 3 | 2 | 1 | 1 | |
| Poa | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |
| Polygonum | 4 | 0 | 0 | 2 | 1 | 3 | 3 | 3 | 3 | 3 | |
| Populus | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | |
| Quercus | 3 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | |
| Rhamnus | 3 | 0 | 0 | 2 | 1 | 2 | 3 | 3 | 3 | 2 | |
| Rubus | 4 | 1 | 0 | 3 | 1 | 3 | 4 | 2 | 4 | 2 | |
| Salix | 3 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 1 | 2 | |
| Silene | 2 | M | 0 | 2 | 0 | 0 | 2 | 2 | 2 | 2 | |
| Solanum | 2 | 0 | 0 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |
| Solidago | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | |
| Sonchus | 2 | NA | NA | M | 0 | 0 | M | 0 | 0 | 0 | |
| Symphyotrichum | 7 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | |
| Trifolium | 2 | M | 0 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |
| Typha | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| Viburnum | 3 | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 1 | 1 | |
| Gymnosperms | Juniperus | 2 | M | 0 | 2 | 2 | 0 | 2 | 2 | 0 | 2 |
| Picea | 2 | NA | NA | 0 | M | 2 | 2 | 0 | 0 | 2 | |
| Pinus | 3 | 1 | 0 | 2 | 1 | 3 | 3 | 3 | 2 | 2 | |
| Monilophytes | Dryopteris | 3 | M | 0 | M | 1 | 1 | M | 1 | 1 | 1 |
| Equisetum | 2 | M | 0 | M | M | 2 | M | 2 | M | M | |
| Lycophytes | Lycopodium | 2 | M | 2 | 2 | M | 2 | 2 | 2 | 2 | M |
| Bryophytes | Brachythecium | 3 | M | 1 | 2 | 2 | 2 | 1† | 3 | 3 | M |
| Dicranum | 2 | NA | NA | 2 | 2 | 2 | M | 2 | 2 | M | |
| Plagiomnium | 2 | NA | NA | M | 2 | 2 | M | 2 | 2 | M | |
| Polytrichum | 2 | M | 0 | 2 | 2 | 2 | M | 2 | 2 | M | |
| No. of species amplified for at least two populations | 58 | 82 | 85 | 88 | 92 | 80 | 92 | 88 | 78 | ||
| Percent species monophyletic (of those amplified and sequenced) | - | 10 | 7 | 43 | 29 | 48 | 56 | 59 | 45 | 44 | |
| Percent species monophyletic (of those attempted) | - | 6.7 | 6.7 | 39.1 | 27.2 | 47.8 | 47.8 | 58.7 | 43.5 | 35.9 | |
Values indicate the number of species for each genus identified as monophyletic with at least 70% bootstrap support. ‘M’ indicates that none of the species in that genus had more than one sample, or that only one species was amplified; ‘NA’: amplifications not attempted in all species for this genus.
†
Low resolution (1 of 3 species) attributed to partial amplification success in this genus, rather than failure to form a monophyletic group.