Table 3. Number of species per genus resolved as monophyletic for each of five proposed multilocus barcoding combinations.
Citation for the proposed combination: | Proposed barcoding combinations | ||||||
rbcL+trnH-psbA | matK+rpoC1+rpoB | matK+rpoC1+trnH-psbA | matK+atpF–atpH+trnH-psbA | matK+atpF–atpH+psbK–psbI | |||
Kress and Erickson [8] | Chase et al. [9] | Chase et al. [9] | Lee et al. [20] | Lee et al. [20] | |||
Major clade | Genus | No. of species surveyed | |||||
Angiosperms | Acer | 5 | 3 | 3 | 3 | 3 | 3 |
Betula | 2 | 0 | 0 | 0 | M | M | |
Cornus | 4 | 4 | 2 | 4 | 4 | 4 | |
Erigeron | 2 | 0 | 0 | 0 | 0 | 0 | |
Eupatorium | 2 | 2 | 2 | 2 | 2 | 2 | |
Lactuca | 2 | 2 | 2 | 2 | 2 | 2 | |
Plantago | 3 | 2 | 3 | 3 | 3 | 3 | |
Poa | 2 | 2 | 2 | 2 | 2 | 2 | |
Polygonum | 4 | 4 | 3 | 4 | 4 | 3 | |
Populus | 4 | 3 | 1 | 2 | 2 | 3 | |
Quercus | 3 | 1 | 1 | 1 | 1 | 1 | |
Rhamnus | 3 | 3 | 3 | 3 | 3 | 3 | |
Rubus | 4 | 3 | 4 | 4 | 4 | 4 | |
Salix | 3 | 2 | 2 | 2 | 2 | 3 | |
Silene | 2 | 2 | 2 | 2 | 2 | 2 | |
Solanum | 2 | 2 | 2 | 2 | 2 | 2 | |
Solidago | 6 | 0 | 0 | 0 | 0 | 0 | |
Sonchus | 2 | 0 | M | M | M | M | |
Symphyotrichum | 7 | 0 | 2 | 2 | 1 | 2 | |
Trifolium | 2 | 2 | 2 | 2 | 2 | 2 | |
Typha | 2 | 0 | 0 | 0 | 0 | 0 | |
Viburnum | 3 | 3 | 2 | 3 | 3 | 3 | |
Gymnosperms | Juniperus | 2 | 2 | 2 | 2 | 2 | 2 |
Picea | 2 | 0 | 0 | 0 | 2 | 2 | |
Pinus | 3 | 3 | 3 | 3 | 3 | 3 | |
Monilophytes | Dryopteris | 3 | 1 | 1 | 1 | 1 | 1 |
Equisetum | 2 | 2 | M | 0 | 0 | M | |
Lycophytes | Lycopodium | 2 | 2 | 2 | 2 | 2 | 2 |
Bryophytes | Brachythecium | 3 | 3 | 3 | 3 | 3 | 3 |
Dicranum | 2 | 2 | 2 | 2 | 2 | 2 | |
Plagiomnium | 2 | 2 | 2 | 2 | 2 | 2 | |
Polytrichum | 2 | 2 | 2 | 2 | 2 | 2 | |
No. of species amplified for at least two populations | 92 | 90 | 92 | 92 | 90 | ||
Percent species monophyletic (of those amplified and sequenced) | 64 | 61 | 65 | 66 | 69 | ||
Percent species monophyletic (of those attempted) | 64 | 60 | 65 | 66 | 67 |
Values indicate the number of species for each genus identified as monophyletic with at least 70% bootstrap support for each multi-locus combination; ‘M’ indicates that none of the species in that genus had more than one sample, or that only one species was amplified and sequenced.