TABLE 2.
EC50 and Emax of IP and cAMP production by FPP, NAG, and LPA in GPR92-expressing cells
CV-1 cells were infected with 100 m.o.i. adenovirus with human GPR92 and stimulated with different concentrations of FPP, NAG, and LPA in 12-well plates. IP and cAMP assays were performed as described under “Experimental Procedures.” Data are expressed as the mean ± S.E. of three independent experiments performed in triplicate assays. The Emax values for NAG and LPA are -fold induction at 10 μm.
|
Ligands |
IP production |
cAMP production |
||
|---|---|---|---|---|
| EC50 | Emax | EC50 | Emax | |
| μm | μm | |||
| FPP | 0.38 ± 0.15 | 4.41 ± 0.56 | 1.46 ± 0.52 | 3.15 ± 0.22 |
| NAG | >50 | 2.01 ± 0.25 | ||
| LPA | 1.03 ± 0.42 | 2.98 ± 0.19 | >50 | 2.44 ± 0.30 |