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. 2008 Jul 25;283(30):20978–20988. doi: 10.1074/jbc.M802588200

FIGURE 2.

FIGURE 2.

p190A-deficient fibroblasts are defective for polarized cell migration. A, F-actin staining (phalloidin) of p190A+/+ and p190A-/- cells 2 h and 6 h post-wounding. The white arrows indicate membrane protrusions oriented toward the migration plane in the wild-type cells. The dashed white line indicates the wound plane. B, a scratch-wounded monolayer illustrating Golgi orientation in p190A+/+ and p190A-/- cells at 6 h post-wounding. Blue, nuclear DAPI staining; red, anti-GM130. The dashed white line indicates the wound edge. The superimposed “bent” white lines correspond to the 120° arc at each cell location that faces the plane of the wound. C, graph depicting the quantification of Golgi orientation in p190A+/+ and p190A-/- cells at 0, 2, 4, and 6 h post-wounding. The dashed horizontal line indicates the 33% value that would be expected with random orientation. 200 cells were counted for each genotype, and graphs depict the mean value obtained from three independent experiments, with error bars representing S.D.